Cux1 (NM_001291238) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CDP; Cutl1; Cux; Cux-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228645 representing NM_001291238 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCAATGTGGGATCGATGTTTCAATATTGGAAGCGCTTTGATTTACAGCAGCTGCAGAGAGAAC TTGATGCCACCGCAACAGTATTGGCAAACAGGCAAGATGAGAGCGAACAGTCCAGAAAGCGGCTCATTGA GCAGAGCCGAGAATTCAAGAAGAACACTCCAGAGGATTTACGCAAGCAGGTAGCACCACTGCTAAAGAGC TTCCAAGGGGAGATTGATGCACTGAGTAAAAGAAGCAAAGAAGCAGAGGCAGCCTTCTTGACTGTGTACA AGAGACTAATTGATGTTCCAGATCCGGTACCAGCCCTGGACGTCGGGCAACAGCTGGAAATAAAAGTGCA GCGTCTACACGACATTGAAACAGAGAACCAGAAACTTAGGGAAACACTAGAAGAGTACAACAAGGAGTTT GCTGAAGTGAAAAATCAAGAGGTTACGATAAAAGCACTTAAGGAGAAAATCCGAGAATACGAGCAGACCC TGAAGAGTCAGGCCGAGACCATTGCTCTGGAGAAAGAGCAGAAGCTACAAAATGATTTTGCAGAGAAGGA GAGAAAGCTGCAAGAGACACAGATGTCCACCACCTCAAAACTGGAGGAAGCTGAGCACAAACTCCAGACT CTGCAAACAGCCCTGGAAAAAACTCGAACAGAATTATTTGACCTGAAAACCAAATATGATGAAGAAACTA CTGCAAAGGCCGATGAGATCGAGATGATCATGACCGACCTTGAACGAGCCAACCAGAGGGCAGAGGTGGC ACAGAGAGAAGCAGAGACTTTAAGGGAACAGCTCTCATCGGCCAACCACTCTCTCCAACTGGCCTCGCAG ATCCAGAAGGCTCCAGATGTGGAGCAGGCCATAGAGGTGCTGACCCGATCCAGCCTAGAAGTAGAGTTGG CTGCCAAAGAGCGGGAGATCGCCCAGCTGGTGGAAGATGTGCAGCGACTCCAGGCCAGCCTCACCAAGCT ACGTGAGAATTCCGCCAGCCAGATCTCACAGCTGGAGCAGCAACTGAATGCCAAGAATAGCACACTCAAA CAACTGGAAGAAAAACTCAAAGGCCAGGCTGACTATGAAGAAGTGAAGAAAGAGCTGAACACCCTGAAGT CCATGGAGTTTGCACCATCGGAGGGAGCAGGGACACAGGACTCTACCAAGCCCCTGGAGGTTTTACTCCT GGAGAAGAACCGCTCGCTGCAGTCCGAGAATGCCACGCTGCGCATCTCCAACAGTGACCTGAGCGGGCGC TGTGCGGAGCTGCAGATCCACCTCACTGAGGCCACAGCCAAGGCTGTTGAGCAGAAGGAGCTGATCGCTC GCTTGGAGCAGGACCTCAGCACCATCCAGTCCATCCAACGGCCTGATGCCGAGGGAGCTTCCGAGCAAGG CCTAGAGAAGATTCCAGAACCCATCAAGGAAGCTACAGCTCTGTTCTATGGACCCTCAATGTCATCCAGT GGGACCCTTCCAGAAGGCCAGGTGGACTCCCTGCTTTCCATCATCTCCAGCCAAAGGGAACGTTTCCGCA CCCGGAACCAAGAGCTGGAAGCCGAGAGCCGCATGGCCCAGCACACCATCCAGGCCCTGCAGAGCGAGCT GGACAGCCTGCGCGCTGACAACATCAAACTCTTTGAGAAGATCAAGTTCCTGCAGAGTTACCCTGGCAGA GGTATCGGCAGTGACGACACGGAGCTGCGATACTCCTCCCAATACGAGGAACGCCTGGACCCTTTCTCCT CCTTCAGCAAGAGGGAGCGGCAGAGGAAGTACCTGGGCCTGAGCCCCTGGGACAAGGCCACACTTGGCAT GGGCCGTCTGATTCTCTCCAACAAGATGGCCCGCACCATCAGCTTCTTTTACACCTTGTTCCTGCACTGC CTGGTCTTTCTGGTGCTGTACAAGCTGGCATGGAGTGAGAGTGTGGAGAGAGACTGTGCTGCCACCTGCG CCAAGAAGTTCGCCGATCATCTGCACAAATTCCACGAGAGTGACAACGGAGCAGCAGCTGGTGACTTATG GCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291238
Insert Size	2037 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291238.1, NP_001278167.1
RefSeq Size	3080 bp
RefSeq ORF	2037 bp
Locus ID	13047
Gene Summary	Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III (PubMed:20510857). Also involved in the control of synaptogenesis (Probable). In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) contains alternate 5' and 3' exon structure, and it thus differs in both UTRs and in the 5' and 3' coding regions, compared to variant 3. The encoded isoform (e) has distinct N- and C-termini and is shorter than isoform c.

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