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TrueMABTM Antibodies - Made against Authentic Protein Antigens

Anti-L1CAM TRUEMAB Antibody Clone OTI2A6

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Specifications Related Products Product Reviews FAQs
Catalog No. Description Amount Price Shipping
TA500728
  • anti-L1CAM mouse monoclonal antibody, clone OTI2A6 (formerly 2A6)
100ul 2700.00 现货 Add to Shopping Cart
TA500728S
  • anti-L1CAM mouse monoclonal antibody, clone OTI2A6 (formerly 2A6)
30ul920现货Add to Shopping Cart
CF500728
  • anti-L1CAM mouse monoclonal antibody, clone OTI2A6 (formerly 2A6)
100 ug4400.002周Add to Shopping Cart
   TrueMABTM Antibodies
* Delivery is in business days
WB(2)
IP(1)
IHC(8)
IF(1)
FC(3)
Also for L1CAM (Locus ID 3897)
Gene NameHomo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1 Synonyms:CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
ImmunogenFull length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone OTI2A6 IsotypeIgG1
Species ReactivityHuman Concentration0.97 mg/ml
Purification Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100, Flow 1:100, IP 2-4ug/mg
BackgroundThe protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
Related Pathway
Hemostasis

* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
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IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500728, Dilution 1:50)
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IF Image
Anti-L1CAM mouse monoclonal antibody (TA500728) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM(RC211601).
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IP Image
Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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FC Image
Flow cytometric analysis of Hela cells, using anti-L1CAM antibody(TA500728),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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FC Image
Flow cytometric analysis of Jurkat cells, using anti-L1CAM antibody(TA500728),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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FC Image
Flow cytometric analysis of living A375 cells, using anti-L1CAM antibody(TA500728, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)
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