KIAA0528 (C2CD5) (NM_001286176) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDP138; KIAA0528
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286176, the custom clone sequence may differ by one or more nucleotides ATGCCAGGGAAGCTGAAGGTGAAAATCGTGGCCGGGCGCCATTTGCCAGTGATGGACCGTGCTAGTGACC TGACTGATGCCTTCGTGGAGGTAAAATTTGGTAATACCACCTTTAAAACAGATGTGTACCTTAAGTCACT CAACCCTCAGTGGAACTCGGAGTGGTTTAAATTTGAGGTGGATGATGAAGACTTACAAGATGAACCTTTA CAGATCACAGTTCTTGACCATGATACTTACAGTGCAAATGATGCCATTGGTAAAGTGTACATTGATATTG ATCCTTTACTGTATAGTGAAGCTGCAACAGTCATCTCAGGATGGTTTCCAATTTATGACACCATACATGG TATCCGTGGGGAAATCAATGTAGTTGTCAAAGTAGACCTCTTCAATGATTTAAATCGATTTAGGCAGTCA TCATGTGGAGTCAAATTCTTTTGCACAACGTCTATTCCAAAATGCTATAGAGCTGTGATAATTCATGGAT TTGTAGAAGAACTTGTGGTCAATGAAGACCCAGAATATCAGTGGATTGATCGAATTCGCACACCAAGGGC ATCAAATGAGGCCAGACAGAGACTCATTTCGTTAATGTCAGGTGAGCTGCAGAGGAAGATTGGCTTGAAA GTACTTGAAATGAGAGGAAATGCAGTTGTGGGGTACTTACAGTGTTTCGATCTGGAGGGCGAGTCTGGGT TAGTGGTGCGAGCCATAGGAACGGCGTGTACTCTGGATAAATTAAGTAGCCCAGCAGCATTCCTTCCTGC ATGTAATTCCCCATCCAAAGAAATGAAGGAGATTCCCTTCAATGAAGATCCCAATCCCAATACTCACTCA TCAGGACCCTCAACCCCTCTGAAAAACCAAACTTATTCCTTTTCACCTTCCAAGTCCTACAGTCGACAGT CCTCTTCTTCTGACACAGATTTGAGTTTGACACCCAAAACTGGAATGGGAAGTGGTAGTGCTGGAAAAGA AGGGGGGCCCTTTAAAGCTCTTTTAAGACAACAGACTCAATCAGCGTTGGAACAGAGGGAATTTCCATTT TTTACCTTGACGGCATTTCCTCCTGGATTCCTTGTACACGTTGGGGGTGTAGTTAGTGCACGTTCTGTGA AGCTTTTGGATCGTATCCACAATCCTGATGAACCAGAAACTCGAGATGCATGGTGGGCAGAAATCAGACA AGAAATAAAATCACATGCTAAAGCATTAGGCTGTCATGCTGTAGTGGGCTACAGTGAATCAACTAGCATC TGTGAAGAGGTCTGCATTTTATCTGCATCTGGCACAGCGGCTGTACTGAATCCTAGATTTCTGCAGGATG GCACCGTGGAAGGCTGTTTGGAACAGAGGCTTGAAGAAAATTTGCCTACACGTTGTGGATTTTGTCATAT ACCATATGATGAACTGAATATGCCATTTCCAGCTCATCTCACATATTGCTATAACTGCAGGAAACAAAAA GTTCCTGATGTTCTGTTTACAACTATAGACCTCCCAACAGATGCAACAGTTATTGGAAAAGGTTGTCTTA TTCAAGCAAGGTTATGTCGCTTAAAAAAGAAAGCACAGGCAGAAGCAAATGCTACAGCTATCAGTAATCT CTTGCCATTTATGGAATATGAAGTGCATACTCAGCTAATGAATAAACTAAAACTCAAAGGAATGAATGCT TTGTTTGGACTAAGAATTCAGATCACAGTGGGTGAAAATATGTTGATGGGCTTAGCGTCTGCCACAGGTG TGTATTTAGCAGCTTTACCAACTCCTGGTGGTATTCAGATTGCTGGGAAGACTCCTAATGATGGCTCATA TGAACAACACATCTCTCATATGCAGAAGAAGATAAATGACACAATTGCTAAAAACAAAGAGTTATATGAA ATCAATCCTCCAGAGATATCTGAAGAGATTATAGGATCACCCATCCCAGAACCTAGGCAACGCTCAAGAC TTCTAAGATCTCAATCAGAAAGCTCGGATGAAGTTACAGAATTAGACCTTTCACATGGGAAAAAAGATGC TTTTGTTTTGGAGATTGATGACACAGATGCCATGGAAGATGTCCATTCTCTACTTACTGATGTTCCTCCT CCTTCAGGCTTTTATAGTTGTAATACAGAAATTATGCCCGGTATAAATAATTGGACCTCTGAAATACAGA TGTTCACTTCAGTAAGAGTAATCAGATTAAGCAGCCTCAACCTGACTAATCAAGCTCTCAATAAGAACTT TAATGATCTCTGTGAAAATTTGCTCAAGAGCCTGTACTTTAAACTACGATCTATGATCCCCTGCTGCCTT TGCCATGTAAATTTTACAGTATCTCTGCCTGAAGATGAATTAATTCAGGTTACAGTCACGGCAGTCGCAA TCACTTTTGACAAAAATCAGGCTTTACAAACCACAAAAACCCCTGTTGAAAAGTCATTGCAAAGAGCCTC AACAGATAATGAAGAACTTTTACAATTTCCACTGGAACTGTGTTCAGATTCACTTCCTTCTCATCCTTTT CCACCAGCTAAAGAACACCTGGAGAGTGCAAGTTCTAACTCAGGTATACCAGCTGCACAGAGAGCAACGT CAGTTGATTACAGTTCCTTTGCAGACAGATGCAGCAGCTGGATAGAGCTCATTAAACTGAAAGCTCAGAC CATAAGGCGTGGATCAATTAAGACAACCATGACAGTTGAAAAAGCAAGTCCAGTGGGTGATGGAAATTTC CGGAATCGTTCTGCTCCACCTTGTGCAAATTCCACAGTTGGGGTTGTTAAGATGACACCTCTTTCTTTTA TTCCTGGAGCAAAAATAACTAAGTATCTTGGGATAATTAACATGTTTTTTATTCGAGAGACTACTTCTCT TCGGGAGGAAGGAGGAGTCAGTGGTTTTCTCCATGCTTTTATTGCTGAAGTGTTTGCAATGGTGAGAGCT CATGTTGCTGCATTAGGAGGGAATGCTGTTGTCTCCTACATAATGAAGCAGTGTGTCTTCATGGAGAATC CAAATAAAAACCAGGCACAGTGTCTTATAAATGTAAGTGGTGATGCAGTGGTTTTTGTTCGTGAATCTGA CTTAGAAGTGGTGTCATCTCAGCAACCTACTACCAACTGCCAGTCATCATGTACTGAAGGCGAAGTTACA ACCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286176
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286176.1, NP_001273105.1
RefSeq Size	4626 bp
RefSeq ORF	3156 bp
Locus ID	9847
UniProt ID	Q86YS7
Gene Summary	Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform a. Variants 1 and 2 encode the same isoform (a).

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