TTLL3 (BC009479) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HOTTL
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009479 edited GGCACGAGGAGTTAATGGAATTTTATCAGCCTTTCCCATTTGTGTAGTCTGGGCAGGTTA GGATTTCCGGGGTGGCTTGAAGGTGGGGCCGGAGGCTTGGGAGGGACCTATGGATATCTG CAGGGAGGGCGTGACCAAGGCTGCCTGGATGACGGCGGGGGATGGTGGAATATATCTCCC CCTGCCCTCTCCACCTGCATGTCCCAGGTCCAGCCCTGCCCAAGTTCTATCATTTCCCCA CAGCGAAGGGGCAGAACTCAGGCACCTCGACACTCAGGTCCAGCGCTGTGAGGACATCCT GCAGCAGCTGCAGGCCGTGGTACCCCAGATAGACATGGAAGGGGATCGCAACATCTGGAT CGTGAAGCCAGGAGCCAAGTCCCGCGGACGAGGCATCATGTGCATGGACCACCTGGAGGA GATGCTGAAGCTGGTGAACGGCAACCCCGTGGTGATGAAGGACGGCAAGTGGGTGGTGCA GAAGTATATTGAGCGGCCCCTCCTCATCTTTGGCACCAAGTTTGACCTCAGACAGTGGTT CCTGGTAACTGACTGGAACCCACTTACCGTGTGGTTCTACCGCGACAGCTATATCCGCTT TTCCACGCAGCCCTTCTCCCTGAAGAACCTGGACAAGTGAGCCCCTCTGCTCGCCTCCCA CGAGCTCCCTGCCTAGTTGGGGAGCTGGCAAGCAAACAGGCGATTACAGTGCAGGGGACT CGCGCAGCAGCGCTAGGCTCCACACACAGACTGCAGGCAGGCAGGCCCTCATCCTGCCAG CTGTCCTTGCATCCAACAGATTTTTTTTAACAGACAGGGGGCGGTCTCACTCTGTTACCT AGGCTGGAGTGCAGTGGCATGGTCACGGCTCACTGTAGCCTTGACTTCCTGTGCTCAAGC AATCCTTCCTCCTCAGCCTCCCAAGTGTCTGAGACCACAGGCATGTACCACAGCTGATTT TTAAGTTTTTTAATGTAGACATGGGGTCTCACTATGTTGCCCAGGTTGGTCTCAAACTCC TGGGCTCAAGCGATCATCCCACCTTGGCTTCCCAAAGTGCTGGGATTACACTATGCCTGG CCCCTCCAACAGATTTGACCGAGTGTGTACCTTGTGCCATGCACTGTTCTGGGCACTTGG GGTATAGCAGTGAACAAAACAAGATCCCAGCTCTCAAGGGCTTACATTCTAGCAGGGAGA AACAGGCAAGCAAGAACACAAGTGAAATTATCTAACAGGTTAAAAGATTATTTGGGCCAG GCGCAGTGGCTCATGCCTGTAATCCAAGCACTTTGGGAGGCCGAGGTGGGTGGATCACCT GAGGTCAGGAGTTCGAGACCAGCCTGACCAATATAGCGAAACCCCGTCTCTACTAAAAAT ATAAAAATTAGCCGGGCGTGTTGGCGGGCGCCAGTAGTCCCAGCTACTCGGGAGGCCGTG ACAGGAGAATTGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAACTGAGATCGTGCCATTG CACTCCAGCCTGGGTGACAGATCAAGGATCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009479 unedited NAAGGACAGTTCACATTTGTAAACGACTCATATAGGCGGCACGCGAATTCGCACGAGAAG TTAATGGAATTTTATCAGCCTTTCCCTTTGTGTAGTCTGGGCAGGTTAAGATTTCCGGGG TGGCTTGAAGGTGGGGCCGGAGGCTTGGGAGGGACCTATGGATATCTGCAGGGAGGGCGT GACCAAAGCTGCCTGGATGACGGCGGGGGATGGTGGAATATATCTCCCCCTGCCCTCTCC ACCTGCATGTCCCAGGTCCAGCCCTGCCCAAGTTCTATCATTTCCCCACAGCGAAGGGGC AGAACTCAGGCACCTCGACACTCAGGTCCAGCGCTGTGAGGACATCCTGCAGCAGCTGCA GGCCGTGGTACCCCAGATAGACATGGAAGGGGATCGCAACATCTGGATCGTGAAGCCAGG AGCCAAGTCCCGCGGACGAGGCATCATGTGCATGGACCACCTGGAGGAGATGCTGAAGCT GGTGAACGGCAACCCCGTGGTGATGAAGGACGGCAAGTGGGTGGTGCAGAAGTATATTGA GCGGCCCCTCCTCATCTTTGGCACCAAGTTTGACCTCAGACAGTGGTTCCTGGTAACTGA CTGGAACCCACTTACCGTGTGGTTCTACCGCGACAGCTATATCCGCTTTTCCACGCAGCC CTTCTCCCTGAAGAACCTGGACAAGTGAGCCCCTCTGCTCGCCTCCCACGAGCTCCCTGC CTAGTTGGGGAGCTGGCAAGCAAACAGGCGATTACAGTGCANGGGACTCGCGCAGCAGCG CTAGGCTCCACACACAGACTGCATGCAGGCAGGCCCTCATCCTGCCAGCTGTCCTTGCAT CCA
Restriction Sites	Please inquire
ACCN	BC009479
Insert Size	1560 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009479.1
RefSeq Size	1560 bp
Locus ID	26140
Gene Summary	Monoglycylase which modifies alpha- and beta-tubulin, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction.[UniProtKB/Swiss-Prot Function]

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