Posts for 3/2019
Tips on Molecular Cloning
Mar 12, 2019
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules. These recombinant molecules are used to direct their replication within host organism (1,2). In this article, we describe the basic molecular cloning technique that helps every scientist in their laboratory on a regular basis. Plasmid based cloning comprises of 4 main steps: Insert DNA preparation Vector...