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DDX4 (Locus ID 54514) Human shRNA

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Specifications Related Products Product Manual FAQs
SKU Format Description Vector Price Availability*  
TR305065 Retroviral plasmids
  • DDX4 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector (Gene ID = 54514). 5µg purified plasmid DNA per construct
  • Non-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.



¥6790 10-14工作日

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* These shRNA constructs were designed to be effective against maximum number of transcriptional variants at this gene locus.

Also for DDX4 (Locus ID 54514)
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Reference Data
RefSeq: NM_024415NM_019039NM_001136034NM_001142549NM_001166533NM_001166534XM_011543495
XM_011543497
Synonyms: MGC111074; VASA; VASA; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; OTTHUMP00000122546; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009].
Performance Guranteed:
OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

 

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