pGFP-V-RS shRNA Vector

CAT#: TR30007

HuSH shRNA GFP Retroviral Cloning Vector (pGFP-V-RS Vector), 5 ug



CNY 2,000.00


货期*
现货

规格
    • 5 ug

Cited in 259 publications.

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经常一起买 (1)
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Control Type Negative
Fluorescent Label GFP
Viral Packaging Retroviral
Feature
  • The HuSH pGFP-V-RS plasmid vector contains both 5' and 3' LTRs of Moloney murine leukemia virus (MMLV) that flank the puromycin marker and the U6-shRNA expression cassette. Upon transient transfection of the plasmids into a packaging cell line, replication deficient viruses can be obtained and used to infect target cells. The puromycin-N-acetyl transferase gene and Kanamycin gene provide selection of antibiotics puromycin and kanamycin, respectively. There is an integrated turboGFP element driven by a cMV promoter to readily verify transfection efficiency.
  • U6_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (259)

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