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OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


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TYMP Over-expression Lysate Product

Specifications Related Products WB Protocol FAQs
Cat. # Description Amount Price Shipping
LY419632 Transient overexpression lysate of thymidine phosphorylase (TYMP), transcript variant 2 100 ug ¥3080.0 19-26工作日
LY500001 Empty vector transfected control cell lysate (HEK293 cell lysate) 100 ug Included for Free
Click here for large pack size of this lysate Join protein application webinar

Also for TYMP (NM_001953)

Expression cell line: Human HEK293T cell line
Expression cDNA Clone: TrueORF Clone RC202170
Epitope tag:C-terminal Myc/DDK*
Detection Antibodies:Anti-DDK Antibody (TA50011)
Accession Number:NM_001953   NP_001944
Other Names:TYMP,ECGF; ECGF1; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; TP
Predicted MW:49.8 kDa
Components:1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
Storage:The lysate is shipped with dry ice. Upon receiving, store the sample at -20°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Preparation:HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5µg TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.
Protein Families: Druggable Genome
Protein Pathways: Pyrimidine metabolismDrug metabolism - other enzymesMetabolic pathwaysBladder cancer
*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

 

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