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4C5 Anti-DDK Monoclonal Antibody

AntiDDK Western Blot Image

OriGene has developed 4C5 anti-DDK antibody for the detection of DYKDDDDK epitope (same epitope as Flag?)

  • Validated for Western blot, immunoprecipitation, immunofluorescence and flow cytometry
  • Higher sensitivity and specificity compared to other vendors’ anti-flag antibodies
  • Purify recombinant proteins with high purities and yields
  • Detect recombinant proteins with low expression levels

4C5 anti-DDK antibody has already been used to

  • Validate the recombinant protein expression from more than 15,000 OriGene TrueORF cDNA clones and VERIFY overexpression lysates
  • Purify more than 5,000 human recombinant proteins from HEK293 cells
  • Purify immunogens under native conditions for the development of TrueMAB antibodies


Order a TrueORF clone, get $100 off anti-DDK (Cat# 50011) or anti-tGFP (Cat# TA150041) antibodies. The clone and the antibody order should be in the same PO to qualify. Click here for bundle promotion code. Offer expires on May 31st, 2011.
SKU Description Data Sheet Price Shipping  
TA50011-100 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody pdf ¥1050.00 现货 Add to Shopping Cart
TA50011-1 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody pdf ¥6060.00 现货 Add to Shopping Cart
TA50011-5 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody pdf ¥17460.00 现货 Add to Shopping Cart
TA150015 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Biotin pdf ¥3021.00 2-3周 Add to Shopping Cart
TA150030 Clone OTI4C5, Anti-DDK (FLAG) Monoclonal antibody, HRP pdf ¥3021.00 4-5周 Add to Shopping Cart

* For bulk antibody package, please contact Tech Support
** Peptide sequence of the DDK-tag (Flag?): N-DYKDDDDK-C
*** Flag? is a trademark of Sigma-Aldrich.

AntiDDK IF Image AntiDDK FC Image AntiDDK DDK Comparison Image
Figure 4. 4C5 anti-DDK monoclonal antibody (TA50011) showed higher sensitivity in the side-by-side comparison with M2 monoclonal antibody from vendor S. Both antibodies were used at 1:2000 dilution against same sample (HSPA9) dilutions.


Western Blot Protocol

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